Studies of low molecular weight RNA from cells infected with adenovirus 2. III. The sequence of the promoter for VA-RNA I.
نویسندگان
چکیده
VA-RNA I is one of the very few RNA species produced in animal cells whose transcriptional initiation site is known precisely. We have analyzed the nucleotide sequence of the DNA preceding the 5' end of VA-RNA I and compared it with known prokaryotic promoters and presumptive eukaryotic promoters.
منابع مشابه
Functional mRNA can be generated by RNA polymerase III.
Eukaryotic cellular mRNA is believed to be synthesized exclusively by RNA polymerase II (pol II), whereas pol I produces long rRNAs and pol III produces 5S rRNA, tRNA, and other small RNAs. To determine whether this functional differentiation is obligatory, we examined the translational potential of an artificial pol III transcript. The coding region of the human immunodeficiency virus type 1 t...
متن کاملVA RNAs from avian and human adenoviruses: dramatic differences in length, sequence, and gene location.
Human adenoviruses encode low-molecular-weight RNAs, so-called VA RNAs, which are transcribed by RNA polymerase III. These RNAs are required for an efficient translation of viral mRNAs late after infection. The genes for the VA RNAs in the genome of CELO virus were mapped and characterized. The results showed a number of surprising differences between CELO virus and human adenovirus type 2 (Ad2...
متن کاملFaithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.
The virus-associated (VA) RNAI gene in human adenovirus 2 DNA has been shown by Wu (Wu, G. J. (1978) Proc. Natl. Acad. Sci. U. S. A. 75, 2175--2179) to be transcribed by RNA polymerase III in a human KB cell-free extract. In the present report we have examined the fidelity of transcription of adenovirus 2 DNA and Xenopus oocyte 5 S DNA templates by RNA polymerase III in extracts derived from cu...
متن کاملPurification, properties, and subunit structure of deoxyribonucleic acid-dependent ribonucleic acid polymerase III from uninfected and adenovirus 2-infected KB cells.
Class III DNA-dependent RNA polymerases have been purified from human KB ceils and from KB cells infected with adenovirus 2 by chromatography on heparin-sepharose, DEAE-cellulose, DEAE-Sephadex, CM-Sephadex, and phosphocellulose and by sedimentation in sucrose density gradients. RNA polymerase III from the two cell sources has been purified approximately 14,500and 32,000-fold, respectively, rel...
متن کاملRNA synthesis in isolated nuclei: in vitro initiation of adenovirus 2 major late mRNA precursor.
We have analyzed the RNA produced in vitro by incubating nuclei from HeLa cells infected with adenovirus serotype 2. Our results show that adenovirus-specific RNA is produced at a linear rate for up to 2.5 hr. Hybridization analysis of RNA produced in nuclei isolated 18 hr after infection indicated that transcription begins at the "late promoter" at map position 16.5. Sequence analysis of the 5...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 252 24 شماره
صفحات -
تاریخ انتشار 1977